The tournament has taken up a new format this season - Wolves are set to play six games in the group stages of this season's competition.
Wolves are in Group C alongside Bournemouth, Norwich City and tonight's opponents Bury, with all sides playing each other home and away.
Morgan Gibbs-White is unavailable for tonight's fixture as he is on international duty with England - Ming Yang-Yang in the squad for the first time since signing for the club in late August.
Squad: Harry Beasley, Joe Young, Oskar Buur Rasmussen, Anthony Breslin, Pedro Goncalves, Hakeem Odoffin, Cameron John, Dan Armstrong, Elliot Watt, Adam Osbourne, Andrew Sealey-Harris, Carlos Heredia, Aaron Collins,Donovan Wilson, Jose Xavier, Ryan Rainey, Sherwin Seedorf, Ming Yang-Yang.