Scott Sellars' side are currently bottom in Group D of the Qualifier Round after narrowly falling to deafeat away at Everton in their opening fixture of the competition.
However, Wolves Under 23s approach Monday night's game in strong form, having defeated Accrington Stanley 4-0 in their last match at Molineux in the Checkatrade Trophy.
Portsmouth shared the points in a 0-0 draw at Norwich City in their first Premier League Cup game at Carrow Road in September.
Monday's game kicks-off at 7pm and tickets are priced at £5 for all supporters.
Squad
Harry Beasley, Anthony Breslin, Harry Burgoyne, Nicu Carnat, Joe Delacoe, Sylvain Deslandes, Ross Finnie, Morgan Gibbs-White, Christian Herc, Connor Johnson, Conor Levingston, Hakeem Odoffin, Will Randall, Connor Ronan, Aaron Simpson, Regan Upton and Donovan Wilson.
